Bush point
The mapping population used in this study (A05141) consisted of 162 F1 progeny derived from a cross between ‘Rio Grande Russet’ (female parent) and ‘Premier Russet’ (male parent). ‘Premier Russet’ is a dual purpose (processing and fresh market) potato variety with significant resistance to the accumulation of reducing sugars after long-term storage at low temperatures . Rio Grande Russet is a high yielding fresh market potato variety . A05141 was developed at the USDA-ARS Small Grains and Potato Germplasm Research Unit (Aberdeen, ID) and was one of the mapping populations phenotyped and genotyped by the Solanaceae Coordinated Agricultural Project (SolCAP) .
Phenotypic analysis
Community tests was basically presented within USDA-ARS, College from Idaho Search and you will Extension Heart (Aberdeen, ID) and Vermont County College Tidewater Research Channel (Plymouth, NC) into the summer seasons off 2010 and you will 2011. New progeny and you will mothers was evaluated in a randomized done block structure with one or two replications regarding ten-slope plots at every location. Vine maturity is actually visually scored towards the a 1–9 level on 95 (95DAP) and you can 120 (120DAP) days immediately following planting, having step 1 = early, 100% vines inside a plot dry, while you are nine = extremely later, complete grow which have buds apparent. Bush growth routine try registered at the beginning of flowering and you will indicated given that step three = erect (> 45° which have ground), 5 = semi-upright (30–45° with soil), and you will eight = distribute (Most document ten: Dining table S5). Verticillium wilt and you will early blight (EB) (Altenaria tenuis) sickness were ranked into a 0–nine size. To have EB, 0 ways zero state and you will 9 > 90% of one’s leaf area affected inside a land. To possess VW, the size are a combined score to own leaf urban area/stem flagging considering the problem (Even more file 10: Desk S5). Plots weren’t inoculated, but Aberdeen fields have highest inoculum off Verticillium from the surface, built up over years. Early blight sometimes appears yearly at the ID and you may NC metropolises as well as which trial the illness was allowed to make however, without fungicides taken out the handle.
Storage conditions
Tuber samples was indeed addressed with Isopropyl Chlorpropham (N-(3-chlorophenyl) carbamate) for a price of twenty two ppm to avoid sprouting and you will kept in the 42 °F and you will 95% relative moisture for 90 days.
Sugar determinations
Sugar levels for the treatments inside demo was basically computed from an excellent 10-tuber decide to try utilising the type Sowokinos mais aussi al. with changes. Tubers was clipped having fun with a passionate Kut Footwear Stringer French fry cutter. 200 g Coral Springs escort off tuber tissue obtained throughout the center from new 10 tubers was macerated during the a keen Acme Juicerator (Acme Gadgets, Spring season Mountain, FL). During processing, tuber cells was clean that have 150 mL away from salt-phosphate boundary (0.05 Yards, pH eight.5) having a last homogenate volume of 275 mL. Sugar density was in fact computed playing with an effective YSI model 2700 Analyzer (Purple Springs Device Co., Inc., Yellow Springs, OH) and you will shown towards a % fresh weight basis.
Bud-end and Stem-end fry color
A strip regarding muscle (step 3.0 cm ? 0.8 cm) taken out of each of the 10 tubers included in the new glucose removal techniques was applied having fry color commitment (10 pieces for every single imitate). Pieces was fried within the canola petroleum from the 191 °C to have 3.5 min. Fry colour are calculated contained in this step three min using a product 577 Photovolt Reflection Meter (design 577, Photovolt Instruments Inc., Minneapolis, MN). A green filter was applied therefore the instrument was calibrated playing with a black colored-hole basic because 0.0% reflectance and you can a light plaque (Cat. No. 26-5Cat. Zero. 26–570-08) since the 99.9% reflectance. Dimensions was basically taken on the new concludes of each and every remove. The reduced the fresh new reflectance measurement, the fresh new black this new fry color and you can vice versa.